Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR
2002
Seify abad Shapouri. M.R., Shahid Chamran University, Ahwaz, Iran | Mayahi , M., Shahid Chamran University, Ahwaz, Iran | Charkhkar, S., Veterinary Organization of Iran, Tehran, Iran | and Assasi, K., Shiraz University, Shiraz, Iran
Fourteen isolates of infectious bronchitis virus (IBV) from Iran in 2001 were typed by a type-specific multiplex RT-PCR. The RT-PCR reaction has been designed to detect and differentiate strains of Massachusetts, D274 and 4/91 (793/B) types. Based on the DNA band produced in RT-PCR, twelve isolates were classified in the type Massachusetts and two isolates (13/2001 and 14/2001) in the type 4/91. The identity of isolate 14/2001, as being from the type 4/91, was also confirmed by sequence analysis of its RT-PCR product. The result of this study shows the presence of at least two types of IBV in Iran.
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