Characterization of Ornithobacterium rhinotracheale Isolates from Commercial Chickens
2001
Banani,M., Razi Vaccine and Serum Reseach Institute. Tehran. Iran. | Pourbakhsh, S.A., Razi Vaccine and Serum Reseach Institute. Tehran. Iran. | Khaki, P., Razi Vaccine and Serum Reseach Institute. Tehran. Iran.
Ornithobacterium rhinotracheale (ORT) is a relatively recently discovered bacterium, which it is associated with respiratory diseases. Tracheal samples from carcasses of 100 poultry flocks submitted to Razi Institute were examined for ORT isolation. All these flocks were affected with respiratory disorders. Colonies of ORT were detected after 24h of incubation and identification of ORT isolates were carried out based on the biochemical and serological characteristics. Fifty-nine isolates from broilers, broiler breeders, and layer flocks were identified as ORT and analyzed for serotyping. The isolates were identified as serotype A, using standard antisera against ORT antigens in rapid slide agglutination, agar gel precipitation and immunodot tests. The identification of the species was confirmed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis of whole cell proteins. High similarity of protein profiles of the isolates was observed. These preliminary results establish that ORT serotype A is associated with outbreaks of respiratory diseases in commercial poultry.
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