Study On Efficacy Of Theilerin In Delayed Hypersensitivity Of Bovine Theileriosies
1999
Hashemi Fesharaki , Reza
Freeze - dried schizont tissue culture antigen of T.annulata (Theilerin) was prepared and its total nitrogen was determined at a level of mg/ml. 292 calves, heifers, milking cows already vaccinate.d against theileriosis or recovered from acute t eileriosis were chosen. They were divided into three groups which respectively received 0.1 ml of theilerin intradermally at normal concentration total nitrogen and also two other dilutions (1.10 and 1.100). Pathological changes such as thickness, erythema, cells infiltration and lymphadenopathy on the inoculation site and also general condition of the animals were checked 48 and 72 hours post inoculation. 62.5% of cattle receiving theilerin at a level of normal concentration showed more then one mm increase in skin thickness at the site of inoculation, the mean value of which come to 3.48 mm. The rest of the animals showed a skin thickness increase of 1 mm which can be considered negligible. The pathological examination of the inoculation site skin showed in filtration of lymphocytes. The results indicated that the test is positive and could be used as an accuracy test for checking the immunity in vaccinated animals provided that the cell-mediated immunity in theileria annulata is defenitly protective
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