Effect of liposome-encapsulated chlorella extract on hypertension in spontaneously hypertensive rats
2009
Takahashi, M., Kagoshima Univ. (Japan) | Shimada, H. | Kitamoto, D. | Takara, K. | Wada, K.
We prepared liposome-encapsulated chlorella extract (LEC) using lecithin via combined mechanochemical method of homogenization and microfluidization. LEC was confirmed to comprise small unilamellar vesicles with a diameter of approximately 150 nm by freeze fracture electron microscopy. The bioactivity of LEC was examined for the suppressive effect of hypertension on blood pressure of spontaneously hypertensive rats (SHR). Continuous 10-week administration of LEC (3.6% (w/w) chlorella extract diet) showed a significant anti-hypertensive effect compared to the control administration for 8 and 10 weeks. Moreover, oral LEC administration showed much higher anti-hypertensive effect compared to non-capsulated extract administration. In addition, the active substance responsible for anti-hypertensive effect in the extract presumed to comprise the peptides was confirmed to have a strong ACE inhibitory activity (ICsub(50) : 0.014 mg/ mL). However, the extract showed a decrease in its ACE inhibitory activity following enzymatic digestion. Consequently, encapsulation for oral administration of LEC prevented digestion of the chlorella extract allowing for its high anti-hypertensive effect on SHR.
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