High-density transportation of the S-type rotifer Brachionus plicatilis
2010
Koiso, M., National Center for Stock Enhancement, Nanao, Ishikawa (Japan). Notojima Station | Kuwada, H. | Hagiwara, A.
We aimed at developing a technique for practical mass transportation of the rotifer Brachionus plicatilis (S-type), based on the existing method used in transporting L-type rotifer. Among the two S-type strains used in Japanese public hatcheries, Yaeyama strain showed significantly higher tolerance to low temperature (5degC) than Okayama strain, thus, we used Yaeyama strain in this study. For high density transportation of rotifers cultured at 25degC, pretreatment cooling at 15degC, is effective. Rotifers were packaged at 30,000 ind./ml in 300-1,000 ml seawater. Thereafter, the rotifers were refrigerated at 5degC. The recovery rate of rotifers after these treatments was about 70% after 2 days of storage. Refrigerated rotifers were less viable, and at least one day recovery period at 25degC is necessary before the rotifers can be used as starter of mass culture or food for fish larvae. Based on these results, we conducted a high-density rotifer transportation at 5degC, and 3 x 10E8 rotifers were transported to other hatcheries in 48 hours using a commercial delivery service. The recovery rates of rotifers at arrival were more than 70%. These results showed that high-density transportation of using Yaeyama S-type rotifer strain is possible by optimizing the processes we established.
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