Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae
2008
Kim, J.S. (Dongbu HiTek Co. Ltd., Daejeon, Republic of Korea) | Roh, J.Y. (Seoul National University, Seoul, Republic of Korea) | Choi, J.Y. (Seoul National University, Seoul, Republic of Korea) | Shin, S.C. (Korea Forest Research Institute, Seoul, Republic of Korea) | Jeon, M.J. (Daegu University, Gyeongsan, Republic of Korea) | Je, Y.H. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
To select entomopathogenic fungi controlling aphids effectively, several isolates were screened against second instars of Myzus persicae nymphs in the glasshouse using conidia suspension at 1.0×10∨5 conidia/ml. Among these isolates, SFB-205 conidia showed the highest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface of M. persicae nymphs, and germination and penetration were observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana species through the comparison of 5.8 s rRNA genes. There were 24 polymorphisms between SFB-205 and the previously reported isolate, B. bassiana ATCC74040 using six kinds of primer combinations in amplified fragment length polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biological control agent for the green peach aphid, M. persicae in the field.
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