Identification of ornithine-lactam converted from arginine in Streptomyces incarnatus NRRL8089
2010
Fukuda, K., Okayama Univ. (Japan). Faculty of Agriculture | Tamura, T. | Inagaki, K,
Sinefungin is a nucleoside antibiotic, in which a molecule of L-ornithine is linked to the 5' end of adenosine through a C-C bond. The antibiotic was isolated from the culture broth of Streptomyces incarnatus. For the purpose of detecting intermediate of sinefungin biosynthesis, resting cell suspensions were incubated with supplemental L-arginine, and L-ornithine. 50mM Arginine was converted to a compound X that has low polarity. 50mM ornithine was not converted and remained in reaction solution. Compound X was purified using HPLC, and analyzed using sup(1)H-NMR and FAB-MS. These analyses showed that a compound X is 'ornithine-lactam' (Mw=114), which has a structure of circularized ornithine. These results indicated that S. incarnatus has an enzyme that converts arginine to ornithine-lactam. Such an enzyme has never been reported, and suggested that it may be relevant to sinefungin biosynthesis.
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