Microwave-assisted extraction of paclitaxel from plant cell cultures
2008
Hyun, J.E. (Kongju National University, Kongju, Republic of Korea) | Kim, J.H. (Kongju National University, Kongju, Republic of Korea), E-mail: [email protected]
A simple and efficient microwave-assisted extraction procedure was developed and optimized for the extraction of paclitaxel from the plant cell cultures of Taxus chinensis. The biomass, immersed in a methanol-water mixture, was irradiated with microwaves in a closed-vessel system. The microwave-assisted extraction was compared with the existing conventional solvent extraction in terms of yield, extraction time, and solvent consumption. The use of microwave energy allows rapid recovery of paclitaxel from biomass and dramatically reduces extraction time and solvent usage compared to conventional solvent extraction. The paclitaxel was completely extracted from biomass by microwave-assisted extraction for 3 min at 50℃, for 6 min at 30℃ and 40℃, respectively.
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