Genetic variability and disturbance of the Japanese common loach Misgurnus anguillcaudatus in Ehime prefecture, Japan
2010
Takagi, M., Ehime Univ., Matsuyama (Japan) | Ohyama, A. | Shimizu, T.
Genetic variability and disturbance of the Japanese common loach Misgurnus anguilicaudatus were surveyed by using 11 samples from 9 wild populations and two artificial stocks, based on 3 micro satellite loci. Investigation of these marker loci of the loach sample showed the difference of genetic variability from each other. The estimated heterozygosity ranged from 0.435 to 0.834. Ratio of observed and estimated heterozygosity was very low in Hokota reservoir samples, fishing item shop and food market. Genetic divergence among the loach samples was observed in the genotype and allele frequencies at the marker loci. Considerable genetic difference was observed between Hokota reservoir samples and the other wild samples. Meanwhile, Genetic distance among Hokota reservoir samples, fishing item shop and food market was very low. The results suggested that the present distribution of the loach in Ehime Prefecture was received several degree of invasion originated from artificial stocks. The micro satellite markers showed a potential as indicators to evaluate genetic variability and to trace genetic changes in Japanese common loach.
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