Estrous cycle judgment of dairy cattle using progesterone concentration kit of raw milk
2010
Tanaka, K., Fukui-ken. Animal Experiment Station, Mikuni (Japan) | Takeuchi, T. | Kondo, M.
The simple diagnosis technic of estrous cycle phase which a dairy farmer himself can judge objectively is effective for the improvement of the reproductive performance of the dairy cattle. So a progesterone concentration kit using raw milk was applied to judge estrous cycle phase. The plate of the kit is cut in 2 connection wells, respectively consist of inspection progesterone standard liquid (1ng/mL) and raw milk sample. The decision of the inspection went by coloring after the 5 minutes of addition 30 minutes color tone. The estrous cycle can be distinguished in three of the following standard by examining its time and color tone. 1) It can be judged estrous phase if a coloring begins in 5 minutes and the same color tone level of the contrast after 30 minutes. 2) It can be distinguished before or after the estrous by 30 minutes if it is thin in comparison with the contrast though it colors doesn't react even if a coloring liquid is thrown and it passes for 5 minutes. 3) It can be judged a luteal phase if a color tone is transparent even if 30 minutes pass after a coloring liquid is thrown.
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