Effect of Production In Vitro Embryo using Boar Frozen Semen
2009
Cho, S.R., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, H.J., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choe, C.Y., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Son, D.S., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choi, S.H., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Son, J.K., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, S.J., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, J.B., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Han, M.H., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea | Jin, H.J., Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Republic of Korea
This study was carried out to investigate the effective genetic resources preservation system using the frozen boar semen. The porcine oocytes were matured for 44 hours in NCSU-23 medium with or without 10% Porcine Follicle Fluid (PFF), 0.5 ㎍/ml porcine FSH, 0.5 ㎍/ml equine LH, 1.0 ㎍/ml 17 β-estradiol (E₂) and 10 ng/ml Epidermal Growth Factor (EGF) under mineral oil at 38.5℃ in humidified atmosphere of 5% CO₂ in air. After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared with mTBM medium for 6 h. Later, set of 50 presumptive zygotes were transferred into 4-well dish (500 μl) of IVC medium. for embryos freezing, slow-freezing and vitrification methods were used as a cryopreservation. Differences among treatments were analyzed using General Linear Model Procedure by SAS Package (version 6.12) differences were considered significant when p less than 0.05. Following IVF and IVC, the rates of cleavage and blastocysts formation were significantly higher (p less than 0.05) in hormone supplemented group than that of hormone-free group (25.7 vs, 12.1). The development rates to cleavage and blastocysts were significantly higher in PZM-5 group than NCSU-23 group (60.3%, 46.6% vs 27.4%, 11.1%). Further improvement was achieved when PZM-5 was supplemented with FBS. Cleavage rates was significantly higher in fresh semen source group than frozen semen (66.7% vs 43.7%). However in blastocysts rates was similar two groups. Post-thaw survival rates of embryos were 1.2% and 2.2% in slow-frezing and vitrification groups, respectively. The results of our study suggest that it is still possible to improve the culture conditions and boar semen cryopreservation for enhance reproductive technology and animal genetic resources conservation.
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