The PLS-CV model for the determination of total crude protein content of forages harvested in may from a permanent meadow (Gradinari; Caras-Severin) [Romania] using NIR spectroscopy
2010
Harmanescu, M., Banat's University of Agricultural Sciences and Veterinary Medicine, Timisoara (Romania) | Moisuc, A.
This paper presents our studies regarding the determination of total crude protein content (%) from forages, using NIR Spectroscopy. The forage samples were harvested in the middle of May 2008 from a permanent meadow situated in Caras-Severin County, near Gradinari. The permanent meadow was organized in ten trials with different doses of organic and mineral fertilizations and five replicates for each trial. The fertilization of permanent meadow was made using: GP1 - unfertilized variant, GP2 - 20t sheep manure, GP3 - 40t sheep manure, GP4 - 60t sheep manure, GP5 - 20t sheep manure + 50 P2O5, GP6 - 20t sheep manure + 50 P2O5 + 50 K2O, GP7 - 20t sheep manure + 50 N + 50 P2O5 + 50 K2O, GP8 - 100 N + 50 P2O5 + 50 K2O, GP9 - 150 N + 50 P2O5 + 50 K2O, GP10 _ (100 + 100) N + 50 P2O5 + 50 K2O. The main species founded on the permanent meadow from Gradinari were: Festuca rupicola and Calamagrostis epigeios. Other species were Antohoxanthum odoratum, Briza media, Poa pratensis, Trifolium arvense, Trifolium medium, Lotus corniculatus and Filipendula vulgaris. The mathematical model (PLS-Cross Validation model) for the determination of total crude protein content (%) by NIR method was performed using the values of reflectance from NIR spectra and those obtained with chemical Kjeldahl method. For the statistical interpretation we used UNSCRAMBLE software. The external calibration was made using forage samples harvested from the same permanent meadow, but in March 2008.
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