Expression profiling of three putative ERF transcription factor genes located at the submergence tolerance locus sub1 in rice (Oryza sativa)
2008
Dang Thi Minh Trang, Philippines Univ. Los Baños, College, Laguna (Philippines)
The major QTL Sub1, located on chromosome 9, substantially contributes to submergence tolerance in rice (Oryza sativa L.). Sequencing of the Sub1 locus in the donor parent FR13A revealed that three ethylene response factors (ERFs) are present in the locus, namely Sub1A, Sub1B and Sub1C, belonging to the B-2 subgroup of the ERF gene family. Gene expression analyses of Sub1A, Sub1B and Sub1C demonstrated that all three genes were induced under submergence. Sub1A was strongly induced in the tolerant accessions and associated with submergence tolerance, whereas Sub1C in RNA was highly accumulated in the intolerant accessions. Sub1B was constitutively expressed in both intolerant and tolerant accessions. A study of 23 rice accessions showed that expression of Sub1B and Sub1C is not always associated with tolerance. This study on the tissue specific expression of Sub1A further showed the best association between the expression of Sub1A and tolerance in the internodes. Preliminary analysis during early plant development suggested that Sub1 did not play a role in submergence tolerance at a very early stage (two to five days after germination). In addition, phenotypic observation suggested that Sub1A started to have an effect on tolerance in six day old seedlings.
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