Genetic linkage map of maize (Zea mays L.) for localization of resistance genes against bacterial stalk rot
2009
Canama, A.O. | Hautea, D.M., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst of Plant Breeding-Crop Science Cluster
Maize (Zea mays L.) is the Philippines' most important crop next to rice. Bacterial stalk rot (BSR) caused by Pectobacterium chrysanthemi pv.zeae Burk., McFad, and Dim. is one of the most important diseases in maize. Improve host resistance provides an efficient method on increasing corn production especially in infested areas. Advances in molecular marker technologies have enabled the utilization of DNA markers as a tool in marker-assisted breeding to develop outstanding varieties of maize with resistance to BSR. Molecular marker-based linkage maps are used for gene localization and isolation and marker-aided selection. Linkage maps facilitate the identification of markers tightly linked to genes of major economic importance such as disease resistance. The authors report the construction of maize linkage maps using simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP) and resistance gene analogs (RGA) polymerase chain reaction (PCR)-based DNA markers. Molecular marker analysis of the BSR-resistant (Y1F62) and BSR-susceptible (P8) parental inbred lines used in the study generated a total of 102 polymorphic SSR, AFLP and RGA markers for map construction. Using 120 F2 progenies from the cross, P8×Y1F62, a linkage map was constructed consisting of 77 mapped markers. Thirty five SSR, 34 AFLP and eight RGA markers were mapped covering a distance of 932.8 cM with approximately 12.1 cM marker density. The integrated linkage map is about 54% of the total map length of maize. This linkage map was successfully used to locate quantitative trait loci (QTL) for bacterial stalk rot resistance genes.
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