Selective precipitation based economical methods for purification of human serum albumin from the out-dated blood bank samples
2010
Nadeem, M.S. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences) | Rauf, A. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences) | Mustafa, M. (Punjab Univ., Lahore (Pakistan). School of Biological Sciences) | Iqbal, M. | Shakoori, A.R.
Based on the studies of solubility of serum albumin in the organic solvents in the presence of trichloroacetic acid and its heat stability in the presence of octanoic acid, two methods have been developed for its purification by selective ammonium sulphate and cold ethanol precipitation. The purification methods used in the present study gave 32% recovery with up to 97% pure final product. The purity and molecular weights of final product was confirmed by SDS-PAGE and gel-exclusion chromatography.
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