Estabilidad genética en plántulas de olivo obtenidas vía embriogénesis somática
2009
Bradaï, F.
This investigation was aimed to study the effects of age and embryogenic line, on the process of somatic embryogenesis (proliferation, maturation and germination) and the genetic stability of plants regenerated in vitro and micropropagated. The regeneration of olive plants by somatic embryogenesis was achieved from fragments of the radicle of the zygotic embryo. The results have shown an increased rate of proliferation depending on the age of cultures and differences in the general appearance of these cultures. It was observed a reduced regeneration capacity in the embryogenic stages of maturation and germination, in cultures maintained in vitro for a long period of time. Nevertheless, and despite their small size, the plants regenerated from old embryogenic cultures have a higher average shoot number. During micropropagation phases (multiplication, rooting and acclimatization) plantlets obtained showed no significant differences in their behaviour. However, the shoots coming from young embryogenic lines show a higher vigour giving rise also to vigorous axillary shoots. Somaclonal variation affects plants of embryogenic cultures of the two ages. Indeed, it was noticed a relationship between the number of copies of a given somatic embryo and the frequency of phenotypic variants observed
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