Potency testing of attenuated live vaccine Lavir-K(R) against classical swine fever | Ispitivanje zastitnog dejstva atenuisane zive vakcine Lavir-K(R) protiv klasicne kuge svinja u eksperimentalnim uslovima
2009
Stojanovic, D., Naucni institut za veterinarstvo Novi Sad, Novi Sad (Serbia) | Plavsic, B., Ministarstvo poljoprivrede, sumarstva i vodoprivrede Republike Srbije, Beograd (Serbia). Uprava za veterinu | Ratajac, R. | Velhner, M. | Prodanov, J. | Petrovic, T. | Vidic, B., Naucni institut za veterinarstvo Novi Sad, Novi Sad (Serbia)
In the paper are presented the results of investigating protective effect of attenuated live vaccine against classical swine fever (CSF) under experimental conditions. The vaccine contains C-strain of CSF. In the Republic of Serbia it is used for systemic immunoprophylaxis. The experiment were carried out on twelve weaner pigs seven weeks of age randomly assigned to two groups consisting of five, and one group consisting of two pigs. Before introducing the experimental protocol of vaccination and artificial infection, the presence of specific antibodies against CSF was controlled by ELISA test, i.e. by serum neutralisation test that proved no presence of antibodies against bovine virus diarrhea (BVD). The pigs in the first group were vaccinated with attenuated live vaccine Lavir-K(R) (Veterinary Institute Zemun a.d., Belgrade) in a 1:40 dilution, and the pigs from the second group were vaccinated in a 1:160 dilution. In the group of animals vaccinated with vaccine Lavir-K(R) in a 1:40 dilution there were no clinical characteristic symptoms of CSF, while in the group vaccinated in a 1:160 dilution two pigs became ill with obvious CSF symptoms. One pig died on day 7 p.v. In the control group, i.e. two non-vaccinated pigs, there was an acute form of CSF and they died seven day after the infection. Calculation of the Protective Dose 50 (PD50) of Lavir-K(R) was done using the equation by Spearman-Kaerber and it was calculated to be 184, and it may be concluded that tested vaccine fulfils the requirements for vaccine potency as laid down in the European Pharmacopoeia and the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
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