Transient expression in tobacco bright yellow 2 cells and pollen grains: a fast, efficient and reliable system for functional promoter analysis of plant genes
2010
Bratic, A.M., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Majic, D.B., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Samardzic, J.T., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Kragl, M.W., Max F. Perutz Laboratories, Wien (Austria) | Maksimovic, V.R., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spacially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this study we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.
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