Biological variability of Zucchini yellow mosaic virus in Serbia | Bioloska varijabilnost virusa zutog mozaika cukinije u Srbiji
2009
Vucurovic, A., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Bulajic, A., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Djekic, I., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Ristic, D., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet | Berenji, J., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Krstic, B., Univerzitet u Beogradu, Beograd - Zemun (Serbia). Poljoprivredni fakultet
In this paper, we present the first analysis of phenotypic variations of Zucchini yellow mosaic virus isoaltes sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelwe isolates were obtained by mechanical inoculation on the Cucurbita pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in the single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian Zucchini yellow mosaic virus isolates and their molecular relationship with Zucchini yellow mosaic virus isoaltes from other parts of the world.
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