Morphological and molecular identification of potato cyst nematode populations in Serbia
2010
Oro, V., Institute for Plant Protection and Environment, Belgrade (Serbia) | Ivanović, Ž., Institute for Plant Protection and Environment, Belgrade (Serbia) | Nikolić, B., Institute for Plant Protection and Environment, Belgrade (Serbia) | Barszi, L., Faculty of Sciences, Novi Sad (Serbia) | Radivojević, M., Faculty of Agriculture, Belgrade - Zemun (Serbia) | Jovčić, B., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
Quarantine species such as potato cyst nematodes Globodera rostochiensis and Globodera pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Sanac, Gojna Gora, and Milatovici were grown on susceptible potato varieties and their morphological differences have been discussed. The avoid ambiguites in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.
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