[Phytohormone content in microstrobiles and microspore-derived callus of Larix sibirica in vitro]
2009
Tretiyakova, I.N. | Ivanitskaya, A.S. | Barsukova, A.V., Russian Academy of Sciences. Siberian Branch, Krasnoyarsk (Russian Federation). The V.N. Sukachiev Inst. of Forest Science | Ivanova, A.N., Siberia Federal Univ., Krasnoyarsk (Russian Federation)
The phenomenon of microspore-derived (MD) embryogenesis is associated with development changeover of male generative cells-microspores from their typical gametophyte one to sporophyte. MD callus and embryoids (embryo-like structures of microsporal origin) of Larix sibirica Ledeb. are inducted by tissue culture. Hormonal balance study of MD larch cultures by ELISA method has shown that of all hormone types, especially cytokinins and abscisic acid, increase significantly in them in compare with start explants (microstrobiles). This probably is connected with active cell divisions and embryoid forming. At this cytokinin increase (in 2 times) and abscisic acid decrease (in 2 times) are observed in MD cultures of plants which have been not damaged by Dasineura rozhkovi Mam. et Nik in compare with the damaged plants. This proves a more intensive growth of intact plants. Phytohormone content in MD callus has been compared with their accumulation in embryos of larch seeds gathered of the intact plants. The conclusion is made that it is necessary to add in nutrient medium auxins (no more than 0.5 milli gram/l) for effective growth of MD cultures and embryoids induction.
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