Genotyping of the bacillus anthracis vaccine strains using the multiloci VNTR-analysis | Генетическое типирование вакцинных штаммов Bacillus anthracis методом мультилокусного VNTR-анализа
2009
Kuznetsovskij, A.V. | Migova, L.V. | Fedorov, Yu.N. | Kibirev, Ya.A. | Vorob'ev, A.A. | Darmov, I.V. | Borisevich, I. V. | Savinykh, A.V. | Gorin, O. V.
Recent years there is a real threat of using biologicals based on the anthrax agent Bacillus anthracis with regard to its relative availability, extreme stability and high lethality. The genotyping variety of 5 known anthracis vaccine strains using 18 variable loci of the chromosomal localization taken from a microbe culture collection of 48 Research Institute of Ministry of Defense was revealed in the research. The stability of the VNTR-loci was shown to be inherited from the B. anthracis strains with common origin and an opportunity of their gene-identification application. The gene profile of each analyzed vaccine strain using every 18 polymorphic loci was determined and the amplification products were sequenced. The variation of electrophoretic mobility of the amplifiers was found to be caused by the presence of the replication elements with various numbers of copies in their structure. By the degree of genetic affinity all strains were divided into 2 clusters A amd B. The strains of European origin have greater genetic similarity and are related to cluster A, while the strains 34F2Sterne and the 2-nd vaccine Tsenkovcky – to cluster B. So, the developed tests allow genotyping and classifying the strains of the anthrax agent
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