Isolation and characterization of bacillus thuringiensis from various habitats in five locations in the Sudan
2009
El-Yass, M.E., University of Khartoum, Khartoum (Sudan). Faculty of Agriculure, Dept. of Botany and Agriculture | Mahdi, A.A., Commission for Biotechnology and Genetic Engineering, Khartoum (Sudan). National Center for Research | Hamza, A.A., Commission for Biotechnology and Genetic Engineering, Khartoum (Sudan). National Center for Research
In a study aiming at the isolation and characterization of Bacillus thuringiensis (B.t.) strains from five localities in the Sudan, biochemical characterization revealed that nine of the 33 isolates exhibited typical B. t. biochemical characteristics, eleven showed deviation in only one biochemical trait and 13 showed deviation in two or more characteristics. Serological characterization of the isolates against three imported reference B.t. subspecies by agglutination tests showed that eight of the isolates were positively agglutinated by the anti-kurstaki serum, three by the anti-israelensis serum and two by the anti-tenebrionis serum. The remaining 20 isolates were untypable by any of the three antisera that were used. The use of these isolates as biocontrol agents against insect pests is discussed
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