Antioxidant defence in porcine granulosa cells exposed to lead in vitro
2009
Capcarová, M., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Kolesárová, A. | Lukáč, N. | Sirotkin, A. | Bárdos, L. | Shubhadeep Roychoudhury
Antioxidants play important role in preventing from free radical-induced cellular damage. In this study the activity of superoxid dismutase (SOD) and total antioxidant status (TAS) of porcine granulosa cells cultured in vitro after lead acetate administration were analyzed using spectrophotometer Genesys 10. Cells were cultured with lead acetate trihydrate Pb(CH3COO)2.3H2O as follows: group Max (5 mg Pb(CH3COO)2.3H2O per 10 ml), group A (2.5 mg per 10ml), group B (0.83 mg per 10ml), group C (0.625 mg per 10ml), group D (0.455 mg per 10ml) and control group without lead addition for 18 hrs. The highest TAS was determined in control group without lead treatment in comparison with other groups (MAX, A, B, C, D). Statistical analyses showed significantly lower value (P less than 0.05) in group B. The activity of SOD was the lowest in control group versus groups treated with lead. After various doses of lead acetate addition porcine granulose cells were capable to survive and produce biochemical substances required for their metabolism. This may be due to activation and involvement of antioxidant mechanisms. The results of present study require further experiments to complete whole task.
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