The use of HPLC in the assessment of the influence of anthropogenic pollution on the properties of humic acids of light soil manured with organic fertilizers
2009
Grzelakowska, A., University of Technology and Life Sciences, Bydgoszcz (Poland) | Banach-Szott, M., University of Technology and Life Sciences, Bydgoszcz (Poland)
Humic substances are naturally developed mixtures of organic compounds, representing various steps of transformation of plant residues. Complex structure of humic substances and their changeability suggest research on new research methods, which would result in the greatest possible amount of information on their structure and transformation. Using of HPLC in HAs analysis allow separating hydrophobic (HOB) from hydrophillic (HIL) fractions. The ratio HIL/HOB can be use as an indicator of degree of transformation of organic matter in soils. The conducted research aimed at assessment of the influence of anthropogenic acidification, salinification and alkalisation on the chosen parameters of humic acids, which to a large extent are responsible for the state of soils. Regardless of the experiments variant, the short-term action of the polluting factors did not influence significantly the properties and transformations of humic substances described with the use of standard parameters. The use of an analytic method, new in the field of humic substances research, namely high-performance liquid chromatography (HPLC) proved significant diversity of the examined humic acids considering their fractional composition.
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