Detection of the eaeA Gene in Escherichia coli from chickens by Polymerase Chain Reaction | Tavuklardan izole edilen Escherichia coli'de eaeA Geninin Polimeraz Zincir Reaksiyonu ile tespiti
2007
Kılıç, A., Veterinary Control and Research Institute, Elazığ (Turkey) | Ertaş, H.B., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Microbiology | Muz, A., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Microbiology | Özbey, G., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Microbiology | Kalender, H., Veterinary Control and Research Institute, Elazığ (Turkey)
anglais. The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined to have this virulence factor.
Afficher plus [+] Moins [-]turc. Bu çalışmada tavuklardan Escherichia coli izolasyonu ve izolatlarda virulens faktörlerinden eaeA geninin Polimeraz Zincir Reaksiyonu ile saptanması amaçlandı. Tavukların iç organlarından kanlı agara ekim yapılarak şüpheli kolonilere uygulanan bazı biyokimyasal testler neticesinde numunelerin % 48 (48/100)'inde E. coli spp. izolasyonu yapıldı. İzolatlardan ekstrakte edilen DNA'lar E. coli'de tespit edilen eaeA geni (virulence geni)'nden türetilen bir çift primer kullanılarak PZR'de amplifikasyona tabii tutuldu. PZR ürünlerinin agaroz jelde değerlendirilmesi neticesinde % 48 (48/100)'inde bu virulens geninin mevcut olduğu belirlendi.
Afficher plus [+] Moins [-]Mots clés AGROVOC
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