Культивирование пастерелл различными способами и их биологические свойства
2010
Medvedev, A.P. | Koshnerova, L.A. | Gvozdev, S.N., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there was realized a cultivation of pasteurellas by different methods in Hottinger broth in 37 deg C temperature in course of 24 hours. Tissue culture properties were studied in accordance with the character of bacteria growth in liquid nutritive media and at the agar surface. Research results showed that in 7 hours after cultivation in media with application of shuttel apparatus and addition of glucose it was possible to obtain the highest quantity of viable pasteurellas with a high degree of virulence. The obtained culture on the basis of obtained results was proposed to be used for the production of virulence antigen.
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