AFLP analysis of cytoplasmic male-sterile line and its maintainer line of pepper | 辣椒细胞质雄性不育系及其保持系的AFLP分析
2010
Luo Xiangdong, Jiangxi Normal University, Nanchang (China), College of Life Sciences | Dai Liangfang, Jiangxi Normal University, Nanchang (China), College of Life Sciences | Chen Jinfeng, Jiangxi Normal University, Nanchang (China), College of Life Sciences
[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP, and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers. [Method] Cytoplasmic male sterility (CMS) line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A, while the specific amplified fragments were recovered or sequenced, and analyzed by BLASTn and TBLASTX in GenBank of NCBI. [Result] A specific fragment AA/CAG169 was obtained from cytoplamic male sterility (CMS) line 21A. Partial DNA sequence of AA/ CAG169 was the series repeated sequence in AFLP marker sequence related to the sex and growth traits of Penaeus monodon. The results of BLASTX and TBLASTX showed that the translation products of this specific fragment had a higher similarity to Solanum demissum putative gag-pol protein, Solanum demissum putative retrotransposon protein and Solanum demissum putative retrotransposon gag protein respectively. [Conclusion] The function of this specific fragement AA/CAG169 is more likely to be closely related to retrotransposon, which indicates that the special fragment is possibly related to the sterility trait.
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