Cloning and sequence analysis of 16S rRNA and COI gene in mitochondrial DNA of Scortum barcoo | 高体革_线粒体DNA16S rRNA和COI基因片断的克隆和序列分析
2010
Zhang Longgang, Fresh Water Fisheries Research Institute of Shandong Province, Jinan (China) | An Li, Fresh Water Fisheries Research Institute of Shandong Province, Jinan (China) | Dong Xuesa, Fresh Water Fisheries Research Institute of Shandong Province, Jinan (China)
[Objective] The aim was to provide molecular biological basis for the researches on the genetic resources, genetic relationship among species and phyletic evolution of S. barcoo. [Method] PCR amplification and sequencing were used to study the 16S rRNA and COI gene fragments. [Result] As for 16S rRNA gene fragments, nucleotide sequences of 791 bp were obtained, and the A, T, G and C contents in this fragment were 31.6％, 21.4％, 20.4％ and 26.7％respectively. As for the COI gene fragments, the size was 631 bp and the A, T, G And C contents were 27.7％, 23.6％, 29.8％ and 18.9％ respectively. Among these two gene fragments, the content of GC was lower than AT, and AT/GC of these two fragments was 1.13 and 1.05 respectively. [Conclusion] The genetic characteristics of gene fragments of 16S rRNA and COI of S. barcoo suggested that the variation in the same species was relatively low. The sequences of 16S rRNA gene in three samples the same, while the sequences of COI gene was also the same, indicating that these two gene of S. barcoo were conservative.
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