Cloning and expression analysis of the high mobility B group genes in Arabidopsis thaliana | 拟南芥高迁移率族蛋白B（HMGB）族基因的克隆及表达分析
2011
Ji Lusha, Liaocheng University, Liaocheng (China), School of Life Science | Wang Hongxia, Liaocheng University, Liaocheng (China), School of Life Science | Guo Shangjing, Liaocheng University, Liaocheng (China), School of Life Science
[Objective] The aim was to better research the function and action mode of High Mobility Group B (HMGB) proteins in higher plants. [Method] At2G33450, At5G23405 and At5G23420 genes of HMGB protein family in Arabidopsis thaliana were cloned by the use of RT-PCR method, and the expression of these three proteins in E.coli and Arabidopsis thaliana were detected by using SDS-PAGE, Northern blot and subcellular localization methods. [Result] The results showed that the molecular weights of the three proteins were 17.5, 17.0 and 27.0 kD respectively, and the expression levels of the proteins in Arabidopsis thaliana were At5G23420＞At5G23405＞At2G33450. In addition, all the three proteins were located in nucleus. [Conclusion] The study will provide a basis for the further research on the biological function of HMGB proteins in higher plants.
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