The optimization of fed-batch fermentation for production of high activity nitrile hydratase by Nocardia sp. | 诺卡氏菌产高活力腈水合酶补料分批发酵工艺的优化_
2010
Huang Weibo, Jiangxi Normal University, Nanchang (China), College of Life Science | Li Changhong, Changjiu Agricultural Chemical Co., LTD, Nanchang (China) | Wang Xiaolan, Jiangxi Normal University, Nanchang (China), College of Life Science
chinois. ［目的］优化诺卡氏菌产腈水合酶的发酵工艺，获得高活力的腈水合酶。［方法］对诺卡氏菌（Nocardia sp.）发酵产腈水合酶过程中菌体生长、pH变化以及葡萄糖消耗对腈水合酶合成的影响进行了分析。［结果］在分批补糖工艺中，使发酵液的葡萄糖浓度维持在1.5～6 g/L，发酵结束时，发酵液酶活达到2 814.3 U/ml，是优化前的8.7倍。［结论］分批补糖工艺增加了菌生长的生物量，大大提高了诺卡氏菌的产酶水平。_
Afficher plus [+] Moins [-]anglais. ［Objective］The aim was to optimize the technology of producing the nitrile hydratase by Nocardia sp. and achieve the high activity nitrile hydratase.［Method］ The effects of the cell growth, pH and glucose consumption on fermenting of Nocardia sp. were studied.［Result］The results showed that maintain the concentation of glucose 1.5-6 g/L for the glucose fed_batch fermentation, the nitrile hydratase activity was 2 814.3 U/ml which was 8.7 times before optimization.［Conclusion］The novel technology could increase the biomass of bacteria growth and greatly improved the enzyme production levels of Nocardia sp. For the glucose fed-batch fermentation._
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