The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds
2011
Xu, Q.L.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Tang, G.W.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Zhang, Q.L.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Huang, Y.K.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Liu, Y.X.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Quan, K.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Zhu, K.Y.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology | Zhang, C.X.,Zhengzhou Coll. of Animal Husbandry Engineering (China). Dept. of Animal Science and Technology
The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. AA genotype conferred higher tenderness (P less than 0.05), muscle marbling score (P less than 0.05) and intramuscular fat content (IMF; P less than 0.05). Thus, the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep.
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