Molecular profiling of bacterial species in the caecum of geese
2011
Liu, B.Y.,Yangzhou Univ. (China). Coll. of Animal Science and Technology | Wang, Z.Y.,Yangzhou Univ. (China). Coll. of Animal Science and Technology | Wang, H.R.,Yangzhou Univ. (China). Coll. of Animal Science and Technology | Hu, P.,Yangzhou Univ. (China). Coll. of Animal Science and Technology | Xu, D.,Yangzhou Univ. (China). Coll. of Animal Science and Technology | Wang, Q.,Yangzhou Univ. (China). Coll. of Animal Science and Technology
The aim of this study was to analyse the microbial diversity in the caecum of geese using a 16S ribosomal RNA gene (rRNA) clone library approach. A total of 160 clones and 124 clones were sequenced and phylogenetically analysed from the contents and mucosa of the caecum of Yang Zhou geese, respectively. Our results indicated that there was a rich variety of bacteria in the caecum contents. Forty-six operational taxonomic units (OTUs) based on a 98% similarity criterion were classified in the contents of goose caecum, as compared to 29 OTUs based on a 97% similarity criterion in the mucosa of goose caecum. The sequences were assigned to 7 and 5 groups in the contents and mucosa of goose caecum, respectively. Contents of goose caecum were dominantly occupied by Clostridia-related species (58.7%) with other abundant sequences being related to Bacteroidetes (26.9%) and Erysipelotrichi (11.2%). Gammaproteobacteria (59.6%) and Clostridia (20.1%) were predominant.
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