Biochemical and molecular markers associated with earliness trait in bread wheat

Ibrahim, Sh.E.; Mandour, A.S.; Ageez, A.A.; Heakal, M.Y.

Biochemical and molecular markers associated with earliness trait in bread wheat

2009

Ibrahim, Sh.E. | Mandour, A.S. | Ageez, A.A. | Heakal, M.Y.

Six parental genotypes of wheat were used that represent a wide range of variability for earliness trait namely; Sids- 4 and Sakha- line (early), Sakha-93and Gemmeiza-7 (medium), Gemmelza-9 and Milan( late). F1 seeds were obtained for five crosses were chosen to make biochemical analysis using SDS - PAGE Banding Pattern. These crosses were (Sids-4 x SakhaQaQ (Sakha-93 x Gemmeiza-7), (Gemmeiza-9 x Milan), (Sids-4xMilan) and (Sids-4 xGemmeiza-7). The obtained results showed that, there are a biochemical and molecular markers associate with earliness trait in wheat. Two bands with molecular weight ranged. from (90.276 to 77.181 KD) in the cross (Sids-4 x Milan), that appeared only in Fl. So, these bands could be used as a selective biochemical marker for earliness trait in wheat. Regarding to the molecular analysis, only one cross (Sid-4 x Milan), was chosen for the wide variation between its parents. RAPDqPCR analysis was carried out using F2 bulked segregate analysis technique. The obtained results showed that, the primer (OPB-10) exhibited only one negative marker with 637bp that appeared in late parent and late bulked F2. In addition, in primer (OPD-05) one positive marker with 1463bp that appeared in early parent and early bulked F2. These two bands could be used as marker for earliness in wheat breeding programs.

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