Evaluation of porcine ACSL4 [Acyl-CoA Synthetase Long-chain 4] gene as candidate gener for meat quality traits in pigs | Evaluación del gen candidato porcino Acyl-CoA Synthetase Long-chain 4 para caracteres de calidad de la carne en cerdos
2011
Corominas, J., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Ramayo, Y., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Castelló, A., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Muñoz, M., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Ibáñez Escriche, N., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Folch, J.M., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària | Ballester, M., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària
Long-chain acyl-CoA synthetase (ACSL) family members catalyse the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, thus, playing an important role in both de novo lipid synthesis and fatty acid catabolism. Previous studies in our group, evaluated the ACSL4 as a positional candidate gene for a quantitative trait loci located in chromosome X in an Iberian x Landrace cross. A c.2645G>A SNP located in the 3'UTR (DQ144454) was associated with the percentages of oleic and monounsaturated fatty acids. The aim of the present work was to evaluate the functional implication of this genetic variant. An expression analysis was performed in 120 individuals with different genotypes for the c.2645G>A polymorphism using the real-time quantitative PCR (SYBR green) method. Differences between genotypes were identified in liver, being the ACSL4 mRNA expression levels higher in animals with the A allele than in animals with the G allele. A SNP genomewide association study with the ACSL4 relative expression levels showed significant positions on chromosomes 2, 6, 11 and 12. Candidate positional genes for ACSL4 regulation were found.
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