Effects of soluble and insoluble fibre dietary levels on bacterial profile in caecotrophes from growing rabbits | Efecto del nivel de fibra soluble e insoluble sobre el perfil bacteriano en cecotrofos de conejos en crecimiento
2011
Rodríguez Romero, N., Universidad Nacional Experimental del Táchira, San Cristóbal (Venezuela) | Abecia, L., Consejo Superior de Investigaciones Científicas, Granada (España). Estación Experimental del Zaidín | Fondevila, M., Universidad de Zaragoza (España). Dept. de Producción Animal y Ciencia de los Alimentos
The effect of neutral detergent fibre (NDF) and soluble fibre (NDSF) on bacterial diversity of caecotrophes from growing rabbits was studied. Four diets were formulated according to two levels of NDF (BI: 370 and AI: 460) and two levels of NDSF (BS: 150 and AS: 180) in a 2x2 factorial structure (n=6). Caecotrophes from 24 rabbits (49 days old) were obtained and bacterial biodiversity (IB) and total bacterial DNA concentration were determined by denaturing gradient gel electrophoresis (DGGE) and real time PCR. Bacterial communities clustered according to their NDSF level, and, within BS, were separated by the NDF level. Shannon Index was not affected by the level of NDF (P>0.05), but increased (P<0.01) with the NDSF from 3.97 to 4.11. Total DNA decreased (P<0.05) with NDF, and the crude protein content of caecotrophes decreased with NDF (P<0.001) and increased with SF (P<0.01). Results indicate that the level of NDSF positively affects concentration and diversity of the caecal microbiota.
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