Micropropagation and field evaluation of Tinospora cordifolia : an important medicinal climber
2010
Mishra, Y. | Usmani, M.G. | Mandal, A.K.
A protocol was developed for clonal propagation of Tinospora cordifoiia through in vitro culture of mature nodal explants. The shoot cultures were established from nodal explants treated. with mercuric chloride (HgCI2) on MS (Murashige and Skoog) medium supplemented with different concentrations of 6-benzyladenine (BA). Among various doses of HgCl2 and BA tested, the nodal segments treated with 0.2% HgCl2for 7 minutes and implanted on MS medium with 5 JIM BA resulted in 100% sprouting and 66% aseptic cultures. Healthy shoots with 4 fold multiplication rate were obtained on MS medium supplemented with 5 JIM BA + 150 JIM glutamine. In vitro regenerated shoots inoculated on 1/2 MS medium enriched with 0.5 JIM indole-3-butyric acid (IBA) resulted in 100% rooting. Rooted plants were successfully hardened and transferred to the field with 100% survival which showed vigorous growth after 6 months. The developed protocol can be used for en masse propagation and conservation of T. cordifolia.
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