Possibility of using Guinea pig to evaluate the efficacy of anthrax vaccine (made in RVSRI) against Bacillus anthracic 17JB strain.
2009
Haji Zadeh, Afshin | Muazzeni Jula, Gholam Reza | Valadan, Majid | Morovvati, Hassan | Ahmadi Niya, A`bd Ol-Reza | Mir Hashemi, Reza | Hatami
According to references; efficacy, potency and stability are considered as essential tests in Quality Control for this vaccine. Potency test of the vaccine is currently carried out on sheep (or goat) using C2 pathogen as challenge strain. Although using natural host assumed as one advantage of this method, there are several disadvantages. One of the main disadvantages is the difficulties in safe containments of sheep and goats during challenge tests since large spaces are required to carry out experiments. High expenses of animals (sheep and goats) is currently spent on challenge tests, is also another disadvantages for. As an alternative procedure, potency test on Guinea pig is also recommended by OIE, FAO and WHO. In this project, considering the current method as a golden test, 80 healthy 400-500 gr. Guinea pigs were divided in 8 groups and vaccinated with 3 consecutive batches of Live Anthrax Spore vaccines and were challenged using B. anthracis strain 17JB. They were well monitored for 21 days and then 50 of them were challenged with 17JB strain and the rest (30) with C2 strain. 3 Guinea pigs were used as control in each group (totally 24 Guinea pigs). After 10 days, all the challenged Guinea pigs survived whereas all the controls died. Our results showed that Guinea pig can be used for potency test of anthrax vaccine as a reliable and feasible laboratory animal instead of sheep by using of 17JB strain.
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