Investigation on peroxidase quantitative changes under in vitro culture of Eucalyptus microtheca
2009
Assareh, M.H., Scientific Board Member, Research Institute of Forests and Rangelands of Iran, Tehran, Iran | Omidi, M., Scientific Board Member, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran | Shabannejad Mamaghani, M., Mamaghani, Master of Science Student, Faculty of Agriculture and Natural Resources, Islamic Azad University of .Karaj Branch | Matinizadeh, M., Member of Scientific Board | Ghamari Zare, A., Research Institute of Forests and Rangelands of Iran, Tehran, Iran | Shahrzad, SH., Research Institute of Forests and Rangelands of Iran, Tehran, Iran
Eucalyptus microtheca F. Muell. is a native Australian species which established successfully at southern subtropical provinces of Iran. Explants from twigs of one years old seedlings of E. microtheca were cultured on modified MS medium (with half-strength KNO3 and NH4NO3), supplemented with 1 mg l-1 NAA + 1 mg l-1 Kin and different concentrations of TDZ. Differentiation obtained with regeneration categories on direct shoot, indirect shoot and somatic embryogenesis. All plantlets were transferred into 0.5 mg l-1 NAA + 0.5 mg l-1 Kin treatment for better shoot and root growth. Peroxidase activity was investigated in plantlets produced from different regeneration types, in order to find out the relationship between the plant growth regulators and the enzyme variation. Peroxidase activity was measured and analyzed in plantlets obtained from different regenerations for determining correlation between peroxidase and different levels of used hormones and also regenerations. However peroxidase activity in direct shoot was low but significant. In indirect shoot with increasing concentration of TDZ enzyme activity increased. This correlation was inverse in somatic embryogenesis so peroxidase activity decreased when TDZ increased. Statistical analysis showed that there was significant difference for peroxidase activity in different regeneration types. The findings of this investigation introduced peroxidase as suitable indicator in respond to various stresses.
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