Cultivar Discrimination of Korean and Chinese Boxthorn (Lycium chinense Mill. and Lycium barbarum L.) using SSR Markers
2009
Chung, J.W., Kongju National University, Yesan, Republic of Korea | Lee, G.A., National Agrobiodiversity Center, National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea | Lee, S.S., Cheongyang Boxthorn Experiment Station, CNARES, Cheongyang, Republic of Korea | Bang, K.H., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Park, C.B., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Park, Y.J., Kongju National University, Yesan, Republic of Korea
This study was undertaken to develop a technique of discrimination using SSR makers in boxthorn cultivars. Forty one boxthorn cultivars, which were collected from Korea and China, were evaluated by 10 SSR markers. Total of 61 alleles were detected, ranging from 3 to 13 with an average of 6.1 alleles per locus. The averages of gene diversity and PIC values were 0.482 and 0.428, with a range from 0.25 (GB-LCM-022 and GB-LCM-087) to 0.83 (GB-LCM-167) and from 0.24 (GB-LCM-022 and GB-LCM-087) to 0.81 (GB-LCM-167), respectively. Five markers out of 10 markers, GB-LCM-022, GB-LCM-075, GB-LCM-104, GB-LCM-167 and GB-LCM-217, were selected as key markers for discrimination in boxthorn cultivars. All of boxthorn cultivars were individually distinguished by the combination of five SSR markers.
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