Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions
2010
Lee, J.W., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Bang, K.H., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Choi, J.J., Panagene Incroporated, Daejeon, Republic of Korea | Chung, J.W., Kongju National University, Yesan, Republic of Korea | Lee, J.H., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Jo, I.H., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Seo, A.Y., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Kim, Y.C., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Kim, O.T., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea | Cha, S.W., National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea
This study describes the identification of Panax species using a peptide nucleic acid (PNA) microarray. P. ginseng, P. quienquefolius, and P. japonicus were distinguished from each other using 5 PNA probes designed based on three single nucleotide polymorphisms (SNPs) detected in internal transcribed spacer (ITS) and 5.8S rDNA regions. Signal intensity comparison between PNA and DNA microarrays revealed that the PNA microarray provides a significantly more stable and specific fluorescent signal intensity than the DNA microarray. Three Panax species identified by the PNA microarray were denoted as bar-code numbers depending on their fluorescent signal patterns of each species using 5 PNA probes (PG-ITS-116, PG-ITS-414-1, PG-ITS-414-2, PG-ITS-425-1, and PG-ITS-425-2). P. ginseng, P. quinquefolius, and P. japonicus were denoted as '11010', '00202' and '00000', respectively. The PNA microarray developed in this study will be useful for legitimizing the distribution of ginseng in domestic and foreign ginseng markets.
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