Development of Protocol for Analyzing Pollinator Insect-mediated Gene Transfer from GM Crop
2010
Baek, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Cho, M.R., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Lee, G.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Oh, Y.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
The aim of this study was to develop a protocol for evaluation of pollinator insect-mediated gene transfer from GM crop to non-GM crop. Insect fauna were investigated in three plots planted with herbicide-resistant transgenic soybean(RRS), G.max L.cv. Taekwang and G. soja. The occurrence number of bee in plot of G. soja and the rate of pollen attachment on the body parts of bee were analyzed at different times. Pollens were collected from the legs of bee trapped in the GM soybean plot. Using the pollen genomic DNA, PCR was performed with GM- and lectin- specific primers to establish the analyzing condition. There was an insignificant difference in the number if insect between the GM soybean plot and non-GM soybean plot. During the flowering time, the rate of bee occurrence was highest at the noon. Since the lectin gene was amplified from the pollen DNA, we confirmed that the harvested pollen was originated from the soybean.
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