Inhibitory Melanogenesis of Bambusae caulis in Taeniam and Profiling of Related Proteins
2010
Lee, C.H., Ajou University, Suwon, Republic of Korea | Kim, S.B., RnD Center, Hankook Cosmetics Co., Ltd., Eumseong, Republic of Korea | Byun, S.Y., Ajou University, Suwon, Republic of Korea
Inhibitory melanogenesis by Bambusae caulis in Teaniam (Phyllostaachys nigra var. henonis Stapf) was studied. Tyrosinase inhibition activities were evaluated with six different extracts. Among them the extract with methanol showed the highest tyrosinase activity inhibition. MTT assay with B16 melanoma showed that the extract was not toxic up to the concentration of 50 ppm. The melanogenesis was clearly inhibited by the extract when it was examined by the melanin content assay in the cell. When the extract was dosed as 10 ppm, the melanogenesis was reduced to 68% in culture medium and 74% in the cell. By the proteome analysis with 2-D electrophoresis, 171 protein spots were found in the control gel and 282 spots were detected in the sample gel. Among 120 spot proteins matched, 12 spots were identified as proteins involved in the melanogenesis mechanism.
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