A core set of microsatellite markers for conservation genetics studies of Korean goral (Naemorhedus caudatus) and its cross-species amplification in Caprinae species
2010
An, J.H., Seoul National University, Seoul, Republic of Korea | Choi, S.K., Seoul National University, Seoul, Republic of Korea | Sommer, Julie, Grewcock Center for Conservation and Research, Omaha's Henry Doorly Zoo, Omaha, NE, USA | Louis Jr, Edward, Grewcock Center for Conservation and Research, Omaha's Henry Doorly Zoo, Omaha, NE, USA | Brenneman, Rick, Grewcock Center for Conservation and Research, Omaha's Henry Doorly Zoo, Omaha, NE, USA | Zemanova, Barbora, Academy of Sciences of the Czech Republic, Konesin, Czech Republic | Hajkova, Petra, Academy of Sciences of the Czech Republic, Konesin, Czech Republic | Park, G.R., Seorak Green Korea United, Sokcho, Republic of Korea | Min, M.S., Seoul National University, Seoul, Republic of Korea | Kim, K.S., Seoul National University, Seoul, Republic of Korea | Lee, H., Seoul National University, Seoul, Republic of Korea
In order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from Korean goral (Naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. Eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four Caprinae species, Japanese serows (Capricornis crispus), Chinese gorals (Naemorhedus goral), Northern chamois (Rupicapra rupicapra) and domestic goats (Capra hircus). In addition, all eleven microsatellites (SY3A, SY12A, SY12B, SY48, SY58, SY71, SY76, SY84, SY84B, SY112, and SY129) satisfied the criteria to be a core set of microsatellites. This core set of microsatellites and cross-species amplification of Korean goral microsatellites were found to be helpful for high-resolution studies for conservation and management of Korean goral and other endangered Caprinae species.
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