Isolation of Bacillus amyloliquefaciens ATC6 Producing Acidic Cellulase
2010
Lee, S.H., Dankook University, Cheonan, Republic of Korea | Chae, J.P., Dankook University, Cheonan, Republic of Korea | Kim, M.J., Dankook University, Cheonan, Republic of Korea | Kang, D.K., Dankook University, Cheonan, Republic of Korea
A bacterium producing acidic cellulase was isolated from pig feces. The isolate, ATC6 strain, was found to be Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus amyloliquefaciens ATC6 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. Optimum pH and temperature for the cellulase activity of the culture supernatant of B. amyloliquefaciens ATC6 were found to be pH 4.5 and 55℃, respectively. More than 80% of its maximum activity was maintained at pH 4.0. The cellulase activity was maintained at temperatures ranging from 35 to 55℃ after 2 h incubation at pH 4.5, whereas it's activity decreased rapidly at 65℃.
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