The resistance of some prebiotics and probiotic bacteria in the stomach model environment | Dažu prebiotiku un probiotisko baktēriju rezistence kuņģa modelī
2010
Bekeris, M., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Grube, M., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Upite, D., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Kaminska, E., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Linde, R., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Pelce, E., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Danilevics, A., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Gavare, M., University of Latvia, Riga (Latvia). Inst. of Microbiology and Biotechnology | Karklina, D., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Food Technology
This study was done to evaluate the resistance of 4% water solution of prebiotics (inulin, maltodextrin, and lactulose) and probiotic bacteria Bifidobacterium lactis Bb-12 and Lactobacillus acidophilus in different stomach model environments. The simplest stomach model environment was an HCl water solution and an oat gruel filtrate with 0.46% of solids at pH 2. Fermentations of oat gruel water environments with or without prebiotics and/or probiotic bacteria were carried out at 37 deg C for 30 or 60 min. In water as well as in oat gruel environments, lactulose and maltodextrin proved to be more resistant than inulin. After 60 min of incubation with raftiline, glucose, and albumin, probiotic bacteria L. acidophilus showed slightly higher viable cell count (VCC) than B. lactis Bb-12. Both cultures had similar good resistance in oat gruel-based stomach model system; however, B. lactis Bb-12 was found to be slightly more resistant than L. acidophilus. The biomass samples of B. lactis Bb-12 and L. acidophilus were discriminated by Fourier transform infrared (FTIR) spectroscopy according to the composition of growth environment in order to evaluate qualitative differences in the carbohydrate composition and quantitative differences in the content of total carbohydrates and proteins in bacterial cells. In the environment with raftiline and albumin, the amount of B. lactis biomass increased nearly twice and the intensity of the absorption band at 1040 cmE-1 increased significantly, which differed from the environment with glucose.
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