Comparison of spectrophotometric methods for assessment of oxidation of milk fat

Antone, U., Latvia Univ. of Agriculture, Jelgava ;Latvia Univ. of Agriculture, Sigulda . Research Inst. of Biotechnology and Veterinary Medicine Sigra; Sterna, V., Latvia Univ. of Agriculture, Sigulda . Research Inst. of Biotechnology and Veterinary Medicine Sigra; Zagorska, J., Latvia Univ. of Agriculture, Jelgava

Comparison of spectrophotometric methods for assessment of oxidation of milk fat

2010

Antone, U., Latvia Univ. of Agriculture, Jelgava (Latvia);Latvia Univ. of Agriculture, Sigulda (Latvia). Research Inst. of Biotechnology and Veterinary Medicine Sigra | Sterna, V., Latvia Univ. of Agriculture, Sigulda (Latvia). Research Inst. of Biotechnology and Veterinary Medicine Sigra | Zagorska, J., Latvia Univ. of Agriculture, Jelgava (Latvia)

Three spectrophotometric methods were compared for the assessment of milk fat oxidation: the AOCS (American Oil Chemists' Society) 2-thiobarbituric acid (TBA) value direct method, the TBA method of Aristova, and the modified method for the estimation of total carbonyl compounds in oils of Endo et al. Two cow milk fat samples – a fresh fat, obtained from fresh milk, and a rancid fat, obtained from a rancid commercial butter by means of high-speed centrifugation (15.871 × g, 30 minutes at 40 deg C) – were analyzed several times in a 22-day period after sample preparation. A positive linear correlation between absorbance and storage time was established in all used methods. The method of Aristova is appropriate for exact determination of oxidation products. This method had rather good R2 value (0.85) of the fresh fat sample results; however, the coefficient of variation (45.78%) showed rather large deviations between parallel analyses. The methods of AOCS and Endo et al. can be used for the assessment of the dynamics of fat oxidation by reporting absorbance values from the assay directly. By these methods rather high R2 values (0.86, 0.93) of the fresh fat sample results were obtained; the coefficients of variation also were satisfactory (10.13%, 11.12%). The last two methods are cheaper and require smaller sample amounts (less than 0.2 g), while the method of AOCS is the most time-consuming method.

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Mots clés AGROVOC

assessment espectrofotometria grasa de la leche keeping quality lait latvia leche letonia lettonie malondialdehyde methode methods milk milk fat milk products oxidation oxydation produit laitier rancidez rancidity recherche research spectrophotometry

Informations bibliographiques

Publié dans

Research for Rural Development. International Scientific Conference Proceedings (Latvia)

Numéro 16/2010 ISSN 1691-4031

Editeur

LLU

Pagination

p. 104-110

D'autres materias

Oxidacion; Malondialdehido; Productos lacteos; Aptitude a la conservation; Aptitud para la conservacion; Investigacion; Matiere grasse du lait; Metodos; Spectrophotometrie; Rancidite

Langue

anglais

Note

Summary (En)

1 table

6 fig., 15 ref.

Type

Conference; Summary

Source

Research for Rural Development 2010. Annual 16th International Scientific Conference Proceedings. Volume No 1, Gaile, Z..- Jelgava (Latvia): LLU, 2010. International Scientific Conference: Research for Rural Development 2010, 16, Jelgava (Latvia), 19-21 May 2010.- p. 104-110

Conférence

International Scientific Conference: Research for Rural Development 2010, 16, Jelgava (Latvia), 19-21 May 2010

Sur AGRIS depuis: 2011-05-15

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Liens

http://www.llu.lv/getfile.php?id=27929 http://www.llu.lv/?ri=5536

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