In silico engineering of biochemical network of Zymomonas Mobilis adaptation for glycerol conversion into bioethanol
2011
Odzina, I., Latvia Univ. of Agriculture, Jelgava (Latvia) | Rutkis, R., University of Latvia, Riga (Latvia) | Kalnenieks, U., University of Latvia, Riga (Latvia)
One of the perspectives of rural development is the production of biofuels. One of the biofuel production problems is a significant quantity (about 10 %) of a by-product – glycerol occurrence. This problem is offered to solve by adaptation of bacteria Zymomonas mobilis, which is notable for ethanol production facilities. To be able to process glycerin into ethanol using Z.mobilis bacteria, the bacteria must be modified, because its natural form cannot process glycerin. At the same time computer modelling analysis is required, to assess specific modification affectivity in interconnection with other processes in bacteria. The computer model, which describes two genes of - bacteria E.Coli GlpF and GlpK insertions and expressions in bacteria Zymamonas mobilis, describes conversion of glycerol into bioethanol in Z.mobilis bacterial cell. Biochemical reactions and the process regulation network are too complicated, to be able to predict the system response without extensive computer modelling by changing any of its components. The first phase of the model creation was creation of a structure model based on biochemical reactions. The second phase of model creation is stoichiometry analysis. The stoichiometry analyzed the possible steady states and reactions flux. Using the databases KEGG, SABIORK, BRENDA, ChEBI are defined reactants, reactions. Stoichiometry analysis of biochemical network of Zymomonas mobilis adaptation for glycerol conversion into bioethanol was created using program COBRA Toolbox.
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