The influence of bioactive compounds extracted from mushroom Agaricus blazei on Jurkat cel [i.e. cells] | Uticaj bioaktivnih komponenata izolovanih iz gljiva Agaricus blazei na Jurkat ćelije
2009
Klaus, A., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Kozarski, M., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Nikšić, M., Poljoprivredni fakultet, Beograd - Zemun (Serbia)
The aim of this experiment was to investigate the effects of different concentrations of Agaricus blazei K and Agaricus blazei B extracts on the growth of Jurkat cells of human lymphocytes. Control samples did not contain mushroom extracts. To all experimental samples extracts were added in the following concentrations: 1 microg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. Bioactive compounds, polysaccharides, beta-glucans were obtained by hot water extraction and alcohol precipitation from dry mushroom carpophores and purified by dialysis. Jurkat cells were exposed to action of mushroom extracts during the incubation period for 72 hours, on 37 deg C, in the atmosphere with 5% CO2. Their number was counted; among all tested concentrations of extracts the most significant influence was observed in concentration of 1 microg/ml Agaricus blazei K, which reduced number of human T lymphocyte Jurkat cells for 39.2%. The extract obtained from Agaricus blazei B reduced the number of human T lymphocyte Jurkat cells for 36.2%.
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