Detection of Ustilago nuda (Jensen) Rostrup in winter barley seed | Detekcija Ustilago nuda (Jensen) Rostrup u semenu ozimog ječma
2011
Ignjatov, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Petrović, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Vujaković, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Taški-Ajduković, K., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Nikolić, Z., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Jovičić, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
Barley is one of the most important cereals grown in our agroecological conditions. The causal agent of loose smut on barley Ustilago nuda (Jensen) Rostrup occurs frequently as varieties susceptible to this pathogen are present in the production. Disease symptoms are manifested on barley head (spike). Parasite is transmitted by seed (seedborne) and stays in the embryo tissue of the infected kernel as dormant mycelium. Recommended method for detection of U. nuda is given by ISTA Rules (method 7-013). In tests, nine samples (weighing 120 g each) of naturally infected barley seed (about 1000 seeds, depending on the absolute mass of seed) were examined, observed and described using a Zeiss microscope with sub stage illumination with magnifi cation range x 40 or higher. Mycelium of the fungus approximately 3 micron thick, golden brown in colour was detected and visible without a stain. The percentage of infected embryos in the examined samples of barley seeds ranged from 0.8% to 5.2%.
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